The role of antibiotics in the treatment of chronic prostatitis: A consensus statement

Practical guidelines for the diagnosis and treatment of chronic prostatitis are presented. Chronic prostatitis is classified as chronic bacterial prostatitis (culture-positive) and chronic inflammatory prostatitis (culture-negative). If chronic bacterial prostatitis is suspected, based on relevant symptoms or recurrent UTIs, underlying urological conditions should be excluded by the following tests: rectal examination, midstream urine culture and residual urine. The diagnosis should be confirmed by the Meares and Stamey technique. Antibiotic therapy is recommended for acute exacerbations of chronic prostatitis, chronic bacterial prostatitis and chronic inflammatory prostatitis, if there is clinical, bacteriological or supporting immunological evidence of prostate infection. Unless a patient presents with fever, antibiotic treatment should not be initiated immediately except in cases of acute prostatitis or acute episodes in a patient with chronic bacterial prostatitis. The work-up, with the appropriate investigations should be done first, within a reasonable time period which, preferably, should not be longer than 1 week. During this period, nonspecific treatment, such as appropriate analgesia to relieve symptoms, should be given. The minimum duration of antibiotic treatment should be 2-4 weeks. If there is no improvement in symptoms, treatment should be stopped and reconsidered. However, if there is improvement, it should be continued for at least a further 2-4 weeks to achieve clinical cure and, hopefully, eradication of the causative pathogen. Antibiotic treatment should not be given for 6-8 weeks without an appraisal of its effectiveness. Currently used antibiotics are reviewed. Of these, the fluoroquinolones ofloxacin and ciprofloxacin are recommended because of their favourable antibacterial spectrum and pharmacokinetic profile. A number of clinical trials are recommended and a standard study design is proposed to help resolve some outstanding issues

Nutrient-Dense Orange-Fleshed Sweetpotato: Advances in Drought-Tolerance Breeding and Understanding of Management Practices for Sustainable Next-Generation Cropping Systems in Sub-Saharan Africa

Almost half of children < 5 years old living in sub-Saharan Africa (SSA) suffer from vitamin A deficiency and 60% suffer from iron deficiency. Thus, there has been a strong commitment to breeding for, promoting awareness of, and delivering adapted pro-vitamin A rich orange-fleshed sweetpotato (OFSP) in SSA during the past two decades and for enhanced iron content since 2014. This review article focuses on major breeding efforts in SSA to enhance the drought tolerance of OFSP and reviews integrated crop management practices for improved and sustained sweetpotato production in SSA farming systems. Under climate change, the frequency and severity of droughts is expected to increase. Technical issues are presented in the context of addressing challenges along the entire value chain to ensure adoption. First, the use of an accelerated breeding scheme reduced the breeding cycle from 8–10 to 4–5 years. Since 2010, 19 drought-tolerant OFSP cultivars have been released in Mozambique, 7 in Malawi, and 2 in South Africa. Moreover, research in four breeding populations using the heterosis exploiting breeding scheme (HEBS) demonstrated that within one breeding cycle of 5 years, clones with significantly higher root yield, abiotic tolerance, host plant resistance to pests and diseases, and early maturity can be produced. In the future, HEBS will be combined with greater use of modern genomic tools, new phenotyping tools, and CRISPR/Cas9-mediated gene editing. Second, beyond genetic enhancements, evidence is presented that using improved crop management systems, existing sweet potato yields can be increased 2–4 times. Current knowledge is reviewed concerning sweetpotato’s role in diverse farming systems, but integrated crop management is clearly under researched. Third, the outlook for drought tolerance breeding indicates that two distinct classes of nutrient-rich cultivars are emerging: (1) Early maturing cultivars (< 4 month growing period) that escape drought but also serve humid environments with small landholding size per capita; and (2) Medium maturing (4–6 month growing period) cultivars that avoid drought, are drought tolerant and exhibit continuous root formation. Increasing commercialization of the crop and climate change will drive demand, and the willingness of farmers to invest in improved sweetpotato crop management

Transcutaneous electrical nerve stimulation using an LTP-like repetitive stimulation protocol for patients with upper limb complex regional pain syndrome: A feasibility study

Introduction This feasibility study aimed to (i) develop a clinical protocol using a long-term potentiation-like repetitive stimulation protocol for transcutaneous electrical nerve stimulation in patients with upper limb complex regional pain syndrome and (ii) develop a research protocol for a single-blind randomised controlled trial investigating the efficacy of transcutaneous electrical nerve stimulation for complex regional pain syndrome. Methods This small-scale single-blind feasibility randomised-controlled trial planned to randomise 30 patients with upper limb complex regional pain syndrome to either a variant of transcutaneous electrical nerve stimulation or placebo transcutaneous electrical nerve stimulation for three weeks. Stimulation comprised 20 pulses over 1 s with a non-stimulation interval of 5 s, a so-called repetitive electrical stimulation protocol following the timing of long-term potentiation. Pain, function and body image were measured at baseline, post-treatment and at three months follow-up. At three months, participants were invited to one-to-one interviews, which were analysed thematically. Results A transcutaneous electrical nerve stimulation protocol with electrodes applied proximal to the area of allodynia in the region of the upper arm was developed. Participant concordance with the protocol was high. Recruitment was below target (transcutaneous electrical nerve stimulation (n = 6), placebo (n = 2)). Mean (SD) pain intensity for the transcutaneous electrical nerve stimulation group on a 0 to 10 scale was 7.2 (2.4), 6.6 (2.8) and 7.8 (1.9), at baseline, post-treatment and at three-month follow-up, respectively. Qualitative data suggested that some patients found transcutaneous electrical nerve stimulation beneficial, easy to use and were still using it at three months. Conclusion Patients tolerated transcutaneous electrical nerve stimulation well, and important methodological information to facilitate the design of a large-scale trial was obtained (ISRCTN48768534). </jats:sec

Β-carotene, iron and zinc content, effect of storage and type of cooking in sweet potato (Ipomoea batatas L) genotypes

El camote (Ipomoea batatas L) es el principal componente en la dieta de millones de personas que viven en países tropicales, y un mejoramiento en el contenido de â-caroteno podría incrementar el suministro de vitamina A para satisfacer la mayor parte de las necesidades diarias de la gente. En el presente estudio, el contenido de â-caroteno (BC), hierro (Fe) y zinc (Zn) fueron determinados en 25 genotipos de camote provenientes de las localidades de La Molina y San Ramón (Perú). Así mismo, los cambios en el contenido de BC en siete genotipos de camote fueron investigados usando diferentes métodos de cocción y tiempos de almacenamiento. El contenido de BC fue medido vía absorbancia a 450 nm e identificado a través del HPLC y el contenido de Fe y Zn fueron determinados a través de técnicas de ICP-OES. Los resultados muestran variación significativa entre genotipo, ambiente y en la interacción genotipo-ambiente. La concentración de BC en las raíces de camote recién cosechado varió entre 7.62 y 18.93 mg/100 g en peso fresco (PF). El contenido de hierro presentó un rango de 0.40 – 0.96 mg/100 g PF y el contenido de zinc presentó un rango de 0.25 – 0.51 mg/100 mg PF. Los genotipos 440442 y 440518 presentaron los niveles más altos de BC en los dos ambientes. Diferencias en el contenido de BC fueron encontrados entre los métodos de cocción y los tiempos de almacenamiento. La concentración de BC de las raíces cocidas por ebullición (15.15 mg/100 g PF) fue mayor que las raíces cocidas por horneado (14.29 mg/100 g PF); sin embargo, no hubo diferencias entre los genotipos 440442 y 420081 por efecto del método de cocción. Asimismo, el contenido de BC se incrementó durante el tiempo de almacenamiento, donde el genotipo 440442 presentó los mayores niveles de BC durante el tiempo de almacenamiento, mientras que los genotipos 440413 y 440513 no presentaron cambios significativos.Sweet potato (Ipomoea batatas L) is the main component in the diet of millions of people living in tropical countries, and an improvement in β-carotene content could increase the supply of vitamin A to meet a greater part of the daily requirements of people. In the present study, the content of β-carotene (BC), iron (Fe) and zinc (Zn) were determined in 25 genotypes of sweet potato from two localities: La Molina and SanRamón. Furthermore, the changes in BC content in seven sweet potato genotypes were investigated using different cooking methods and storage times. The content of BC was measured via absorbance at 450 nm and identified through HPLC while Fe and Zn content were determined through ICP-OES techniques. The results show significant variation between genotype, environment and in the genotype-environment interaction. BC content in freshly harvested raw root ranged from 7.62 to 18.93 mg /100 g in fresh weight (PF), iron content ranged from 0.40 to 0.9 6mg/100 g PF and the zinc content ranged from 0.25 to 0.51 mg/100 mg PF. Genotypes 440442 and 440518 showed the highest levels of BC in bothlocalities. Differences in BC content were found among cooking methods and storage times. BC concentration in boiled roots (15.15 mg/100 g PF) evaluated was higher than in baked roots (14.29 mg/100 g PF); however, without differences between genotypes 440442 and 420081 due to the cooking methods. BC content of root increased with storage time, where genotype 440442 showed the highest levels of BC during storage time, while genotypes 440413 and 440513 did not differ during storage time

Bodenzustandserhebungen: Wie beeinflussen Standort und Nutzung den Kohlenstoffvorrat in Wald- und Agrarböden?

Die Thünen-Institute für für Waldökosysteme und für Agrarklimaschutz koordinieren die bundesweiten Bodenzustandserhebungen (BZE) in Wald- und Agrarböden. Die BZE haben unterschiedliche Ausgangsbedingungen, jedoch das gemeinsame Ziel, aktuellen Zustand und Änderungen der Bodenkohlenstoffvorräte an rund 5000 Probenahmepunkten zu erfassen. Ergebnisse zum Einfluss von Klima, Nutzung und Bodenmanagement dienen zum einen der Entwicklung zu einer nachhaltigen Bodennutzung und zum anderen der Verbesserung der Emissionsberichterstattung nach UN-Klimarahmenkonvention und EU-Regelungen.Nach aktuellem Stand der qualitätsgesicherten Datenbanken beider BZE sollen Bodenkohlenstoffvorräte verschiedener Bodennutzungsarten (Nadelwald, Laubwald, Mischwald, Acker, Dauergrünland, Grünland-Wechselwirtschaft) für mineralische und organische Ober- sowie Unterböden ausgewertet und die Ergebnisse entsprechend präsentiert werden. Ferner soll eine mögliche Stratifizierung der Ergebnisse nach Bodentypen und Bodenklimaräumen geprüft werden. Ziel hierbei ist es, räumliche Datencluster zu bilden, die stabile Ergebnisse zu Zwecken der Berichterstattung und Entscheidungsfindung liefern und gleichzeitig repräsentativ bleiben

Distinct Impacts of Eda and Edar Loss of Function on the Mouse Dentition

The Eda-A1-Edar signaling pathway is involved in the development of organs with an ectodermal origin, including teeth. In mouse, mutants are known for both the ligand, Eda-A1 (Tabby), and the receptor, Edar (Downless). The adult dentitions of these two mutants have classically been considered to be similar. However, previous studies mentioned differences in embryonic dental development between EdaTa and Edardl-J mutants. A detailed study of tooth morphology in mutants bearing losses of functions of these two genes thus appears necessary to test the pattern variability induced by the developmental modifications. 3D-reconstructions of the cheek teeth have been performed at the ESRF (Grenoble, France) by X-ray synchrotron microtomography to assess dental morphology. The morphological variability observed in EdaTa and Edardl-J mutants have then been compared in detail. Despite patchy similarities, our detailed work on cheek teeth in EdaTa and Edardl-J mice show that all dental morphotypes defined in Edardl-J mice resolutely differ from those of EdaTa mice. This study reveals that losses of function of Eda and Edar have distinct impacts on the tooth size and morphology, contrary to what has previously been thought. The results indicate that unknown mechanisms of the Eda pathway are implicated in tooth morphogenesis. Three hypotheses could explain our results; an unexpected role of the Xedar pathway (which is influenced by the Eda gene product but not that of Edar), a more complex connection than has been appreciated between Edar and another protein, or a ligand-independent activity for Edar. Further work is necessary to test these hypotheses and improve our understanding of the mechanisms of development

Casimir force in O(n) lattice models with a diffuse interface

On the example of the spherical model we study, as a function of the temperature $T$, the behavior of the Casimir force in O(n) systems with a diffuse interface and slab geometry $\infty^{d-1}\times L$, where $2<d<4$ is the dimensionality of the system. We consider a system with nearest-neighbor anisotropic interaction constants $J_\parallel$ parallel to the film and $J_\perp$ across it. The model represents the $n\to\infty$ limit of O(n) models with antiperiodic boundary conditions applied across the finite dimension $L$ of the film. We observe that the Casimir amplitude $\Delta_{\rm Casimir}(d|J_\perp,J_\parallel)$ of the anisotropic $d$-dimensional system is related to that one of the isotropic system $\Delta_{\rm Casimir}(d)$ via $\Delta_{\rm Casimir}(d|J_\perp,J_\parallel)=(J_\perp/J_\parallel)^{(d-1)/2} \Delta_{\rm Casimir}(d)$. For $d=3$ we find the exact Casimir amplitude $\Delta_{\rm Casimir}= [ {\rm Cl}_2 (\pi/3)/3-\zeta (3)/(6 \pi)](J_\perp/J_\parallel)$, as well as the exact scaling functions of the Casimir force and of the helicity modulus $\Upsilon(T,L)$. We obtain that $\beta_c\Upsilon(T_c,L)=(2/\pi^{2}) [{\rm Cl}_2(\pi/3)/3+7\zeta(3)/(30\pi)] (J_\perp/J_\parallel)L^{-1}$, where $T_c$ is the critical temperature of the bulk system. We find that the effect of the helicity is thus strong that the Casimir force is repulsive in the whole temperature region.Comment: 15 pages, 3 figure

Critical Casimir forces and adsorption profiles in the presence of a chemically structured substrate

Motivated by recent experiments with confined binary liquid mixtures near demixing, we study the universal critical properties of a system, which belongs to the Ising universality class, in the film geometry. We employ periodic boundary conditions in the two lateral directions and fixed boundary conditions on the two confining surfaces, such that one of them has a spatially homogeneous adsorption preference while the other one exhibits a laterally alternating adsorption preference, resembling locally a single chemical step. By means of Monte Carlo simulations of an improved Hamiltonian, so that the leading scaling corrections are suppressed, numerical integration, and finite-size scaling analysis we determine the critical Casimir force and its universal scaling function for various values of the aspect ratio of the film. In the limit of a vanishing aspect ratio the critical Casimir force of this system reduces to the mean value of the critical Casimir force for laterally homogeneous ++ and +- boundary conditions, corresponding to the surface spins on the two surfaces being fixed to equal and opposite values, respectively. We show that the universal scaling function of the critical Casimir force for small but finite aspect ratios displays a linear dependence on the aspect ratio which is solely due to the presence of the lateral inhomogeneity. We also analyze the order-parameter profiles at criticality and their universal scaling function which allows us to probe theoretical predictions and to compare with experimental data.Comment: revised version, section 5.2 expanded; 53 pages, 12 figures, iopart clas

Finite-size effects in the spherical model of finite thickness

A detailed analysis of the finite-size effects on the bulk critical behaviour of the $d$-dimensional mean spherical model confined to a film geometry with finite thickness $L$ is reported. Along the finite direction different kinds of boundary conditions are applied: periodic $(p)$, antiperiodic $(a)$ and free surfaces with Dirichlet $(D)$, Neumann $(N)$ and a combination of Neumann and Dirichlet $(ND)$ on both surfaces. A systematic method for the evaluation of the finite-size corrections to the free energy for the different types of boundary conditions is proposed. The free energy density and the equation for the spherical field are computed for arbitrary $d$. It is found, for $2<d<4$, that the singular part of the free energy has the required finite-size scaling form at the bulk critical temperature only for $(p)$ and $(a)$. For the remaining boundary conditions the standard finite-size scaling hypothesis is not valid. At $d=3$, the critical amplitude of the singular part of the free energy (related to the so called Casimir amplitude) is estimated. We obtain $\Delta^{(p)}=-2\zeta(3)/(5\pi)=-0.153051...$, $\Delta^{(a)}=0.274543...$ and $\Delta^{(ND)}=0.01922...$, implying a fluctuation--induced attraction between the surfaces for $(p)$ and repulsion in the other two cases. For $(D)$ and $(N)$ we find a logarithmic dependence on $L$.Comment: Version published in J. Phys. A: Math. Theo

The translation, validity and reliability of the German version of the Fremantle Back Awareness Questionnaire

Background: The Fremantle Back Awareness Questionnaire (FreBAQ) claims to assess disrupted self-perception of the back. The aim of this study was to develop a German version of the Fre-BAQ (FreBAQ-G) and assess its test-retest reliability, its known-groups validity and its convergent validity with another purported measure of back perception. Methods: The FreBaQ-G was translated following international guidelines for the transcultural adaptation of questionnaires. Thirty-five patients with non-specific CLBP and 48 healthy participants were recruited. Assessor one administered the FreBAQ-G to each patient with CLBP on two separate days to quantify intra-observer reliability. Assessor two administered the FreBaQ-G to each patient on day 1. The scores were compared to those obtained by assessor one on day 1 to assess inter-observer reliability. Known-groups validity was quantified by comparing the FreBAQ-G score between patients and healthy controls. To assess convergent validity, patient\u27s FreBAQ-G scores were correlated to their two-point discrimination (TPD) scores. Results: Intra- and Inter-observer reliability were both moderate with ICC3.1 = 0.88 (95%CI: 0.77 to 0.94) and 0.89 (95%CI: 0.79 to 0.94), respectively. Intra- and inter-observer limits of agreement (LoA) were 6.2 (95%CI: 5.0±8.1) and 6.0 (4.8±7.8), respectively. The adjusted mean difference between patients and controls was 5.4 (95%CI: 3.0 to 7.8, p\u3c0.01). Patient\u27s FreBAQ-G scores were not associated with TPD thresholds (Pearson\u27s r = -0.05, p = 0.79). Conclusions: The FreBAQ-G demonstrated a degree of reliability and known-groups validity. Interpretation of patient level data should be performed with caution because the LoA were substantial. It did not demonstrate convergent validity against TPD. Floor effects of some items of the FreBAQ-G may have influenced the validity and reliability results. The clinimetric properties of the FreBAQ-G require further investigation as a simple measure of disrupted self-perception of the back before firm recommendations on its use can be made